A typical sex identification problem is facing biologists in groupers. A newly developed method by the fish sex change expert, Alam Mohamed at Sesoko Station, Japan, is an accurate and reliable determinate of the sex of live groupers using 5–10 mg gonadal tissue samples, harvested using non-lethal gonadal biopsy.

Alam’s fish survive the operation and there is no serious infections resulting from the surgery. Alam’s method is therefore quickly spreading around the world.